Sigma-Aldrich
1,4-Dithio-Dl-Threitol 99% (RT) 5 grams
- Part Number:
- MIL-43819-5G
- Lead Time:
- 2-4 weeks
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- each
- Size:
- 5 grams
- Style:
- chemical
- DOT Rated:
- HazMat Transport Fee Required
Bulk discount rates
Automatically save when you buy in bulk.
| Buy 6 - 11 | and get 10% off |
| Buy 12 or above | and get 15% off |
Description
1,4-Dithio-Dl-Threitol ≥99.0% (RT) 5 Gram
Synonym: threo-1,4-Dimercapto-2,3-butanediol, DTT, Cleland's reagent
- CAS Number 3483-12-3
- Linear Formula HSCH2CH(OH)CH(OH)CH2SH
- Molecular Weight 154.25
- Beilstein/REAXYS Number 1719757
- EC Number 222-468-7
- MDL number MFCD00004877
- PubChem Substance ID 329756409
- NACRES NA.21
| Assay | ≥99.0% (RT) |
| Impurities | ≤0.3% 4,5-dihydroxy-1,2-dithiane |
| mp | 40-43°C |
| 41-44°C (lit.) | |
| solubility | H2O: 50 mg/mL, clear, colorless |
| H2O: freely soluble | |
| acetone: freely soluble | |
| chloroform: freely soluble | |
| diethyl ether: freely soluble | |
| ethanol: freely soluble | |
| ethyl acetate: freely soluble | |
| anion traces | sulfate (SO42-): ≤50 mg/kg |
| cation traces | Ca: ≤10 mg/kg |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Na: ≤250 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| storage temp. | 2-8°C |
Application
DL-Dithiothreitol is a pivotal component used in the preparation of buffer which in turn is utilized in the platelet cytosol preparation. It is also important in isolation of RNA by using guanidinium salts and homogenisation of tissue and cells.
An excellent Reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'²-bis(acryloyl)cystami‹ne to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Biochem/physiol Actions
DL-Dithiothreitol denatures proteins by reducing the disulphide bonds and reducing them to SH groups. It also prevents intramolecular and intermolecular disulfide bond formation between cysteine residues of proteins.
GHS07
Signal word Warning
Hazard statements H302-H412
Precautionary statements P264-P270-P273-P301 + P312 + P330-P501
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves
RIDADR UN 3335
WGK Germany 3
RTECS EK1610000
Flash Point(F) 235.4°F
Flash Point(C) 113°C
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
Most chemicals cannot be expedited.
This item has a Haz-Mat shipping fee built into the price. Fee is applicable only to the first item purchased. Additional HazMat items will be discounted automatically in the shopping cart. HazMat items always require special DOT handling and in rare cases may incur additional fees not levied prior to checkout. Request a quote for the most accurate pricing.
WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.