Sigma-Aldrich

Acrylamide For Electrophoresis 99% HPLC Powder 500 gram

Part Number:
MIL-A3553-500G
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2-4 weeks
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Size:
500 grams
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Price: $241.48

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Description

Acrylamide For Electrophoresis ≥99% HPLC Powder 500 Gram

Synonym: 2-Propenamide, Acrylic acid amide

  • CAS Number 79-06-1
  • Linear Formula CH2=CHCONH2
  • Molecular Weight 71.08
  • Beilstein/REAXYS Number 605349
  • EC Number 201-173-7
  • MDL number MFCD00008032
  • eCl@ss 39031205
  • PubChem Substance ID 24890783
  • NACRES NB.22
grade for electrophoresis
vapor density 2.45 (vs air)
vapor pressure 0.03 mmHg ( 40 °C)
assay ≥99% (HPLC)
form powder
impurities ≤0.005% Acrylic acid
  ≤0.005% Insoluble in water
conductivity ≤10 μmho (35% solution)
bp 125 °C/25 mmHg (lit.)
mp 82-86 °C (lit.)
solubility water: soluble 2.5 g/10 mL, clear, colorless
cation traces Fe: ≤1 ppm
  Mg: ≤3 ppm
  heavy metals (as Pb): ≤5 ppm
SMILES string NC(=O)C=C
InChI 1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)
InChI key HRPVXLWXLXDGHG-UHFFFAOYSA-N

General description

Acrylamide functions as a quencher of tryptophan fluorescence. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.

Application

Acrylamide has been used in the preparation of polyacrylamide gels.

GHS06, GHS08

Signal word Danger
Hazard statements H301-H312 + H332-H315-H317-H319-H340-H350-H361f-H372
Precautionary statements Missing Phrase - N15.00950417-P201-P260-P280-P305 + P351 + P338-P308 + P313
Personal Protective Equipment Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
RIDADR UN 2074 6.1 / PGIII
WGK Germany 3
RTECS AS3325000
Flash Point(F) 280.4 °F
Flash Point(C) 138 °C

Documents 

Warnings

Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.

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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning

Please note: This product is not returnable.

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MPN:
A3553-500G