Sigma-Aldrich
Minimum Essential Medium Eagle with Earle's Salts L-Glutamine 1 Liter, case/6
- Part Number:
- MIL-M4655-6X1L
- Lead Time:
- 2-3 weeks
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- 6
- Size:
- 1 liter / 32 ounce
- Style:
- chemical
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Description
Minimum Essential Medium Eagle With Earle′S Salts L-Glutamine 1 Liter Case/6
Synonym: MEM
- NACRES NA.75
| Quality Level | 300 |
| sterility | sterile-filtered |
| form | liquid |
| application(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| components | HEPES: noNaHCO3: yesL-glutamine: yesEarle’s salts (5% CO2): yessodium pyruvate: nophenol red: yes |
| shipped in | ambient |
| storage temp. | 2-8°C |
General description
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle’s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks’ or Earle’s salts has broadened the usefulness of this medium.
Application
Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.