Research Antibiotics, Tetracycline Hydrochloride, EP/USP, 100 grams

Research Antibiotics, Tetracycline Hydrochloride, EP/USP, 100 Grams

Cas#: 60-54-8

Synonyms: tetracycline, 60-54-8, Deschlorobiomycin, Tetracyclinum

Molecular Formula: C22H24N2O8

Tetracycline is a broad-spectrum antibiotic produced from chlortetracycline. It has a role as an antibacterial drug, an antiprotozoal drug, an antimicrobial agent, and a protein synthesis inhibitor. Science mechanist studies have proven that tetracyclines may exert benefit in osteoarthritis progression: matrix metalloproteinase inhibition, immunomodulation, and nitric oxide synthase inhibition.

Tetracycline Hydrochloride, EP/USP, 100 grams. Tetracycline is a light-sensitive bacteriostatic polyketide antibiotic frequently used in a wide range of in-vitro cell culture applications. Tetracycline (achromycin) is a first-generation tetracycline antibiotic and was first identified in 1953 by Chemist Lloyd Conover's team at Pfizer, in collaboration with R.B. Woodward of Harvard University. Tetracycline is a naturally occurring antibiotic from S. aureofaciens, S. rimosus, and S. viridofacien that shows wide ranging activity against both gram-negative and gram-positive bacteria.

Tetracycline is a protein synthesis inhibitor. Tetracycline bind the 30s ribosomal subunit, preventing the aminoacyl-tRNA from attaching to the A site. Consequently, protein synthesis is inhibited. Resistance to tetracycline arises from loss of cell wall permeability, tetracycline efflux, ribosome protection and tetracycline modification.

Tetracycline is used to study transcriptional activation. Knowledge of tetracycline led to the development of a popular inducible expression system in eukaryotic cells known as Tet-Off and Tet-On. Tetracycline is also used in multidrug resistance studies and in cell culture applications as a selective agent. Additionally, it promotes expression of the P450 proteins.

We offer 3 forms of tetracycline: tetracycline HCL (TOK-T004), tetracycline, USP (TOK-T051), and tetracycline, EP (TOK-T016). Tetracycline, USP and tetracycline, EP are sparingly soluble in aqueous solution at 0.231 mg/mL. Tetracycline HCl, is slightly soluble in aqueous solution at 10mg/mL.

Tetracycline, USP (T051) conforms to United States Pharmacopeia specifications.

• Category: Microbiology
• CAS Number: 60-54-8
• Molecular Formula: C₂₂H₂₄N₂O₈ • xH₂O
• Mechanism of Action:
  Tetracycline inhibits bacterial growth in gram-positive and gram-negative bacteria by disrupting codon-anticodon interactions at the ribosome, thus blocking protein synthesis. Specifically, tetracycline binds to a single site on the 30S ribosomal subunit and inhibit protein synthesis by blocking the attachment of charged aminoacyl-tRNA to the A site on the ribosome. Thus, they prevent introduction of new amino acids to the nascent peptide chain. Mammalian cells are not vulnerable to the effect of tetracycline as these cells contain no 30S ribosomal subunits so do not accumulate the drug.
• Storage Conditions: 2-8°C
• Tariff Code: 2941.30.0000
• Spectrum: Tetracycline is a broad spectrum antibiotic, effective against both gram-positive, gram-negative bacteria, and mycoplasma. A few species of bacteria display intrinsic resistance to tetracycline, including Pseudomonas aeruginosa. Acquired (as opposed to inherent) resistance has proliferated in many pathogenic organisms and greatly eroded the versatility of Tetracycline derivatives. Resistance amongst Staphylococcus, Streptococcus, Neisseria gonorrhoeae, and members of the Enterobacteriaceae is now quite common. Tetracyclines show activity against protozoan parasites as well.

Plant Biology Applications

Tetracycline has shown to suppress aster yellows disease symptoms on China Aster and Chrysanthemum plants. Tetracyclines are sprayed onto fruit trees and other plants to treat infection by Erwinia amylovora, injected into palm trees to treat mycoplasma infections (lethal yellow), and used to control infection of seeds by Xanthomonas campestris (black rot). Multiple applications of tetracycline resulted in symptomless plant growth.

Specifications

• Form: powder
• Appearance: Yellow crystalline powder
• pH: 3.5 - 7.0
• Assay: Not less than 975 µg/mg (on dried basis)
• Loss on Drying: Not more than 13.0%
• Heavy Metals: Not more than 0.005%
• Impurities: Not more than 2.0% (4-epianhydrotetracycline)

References

National Center for Biotechnology Information (2022). PubChem Compound Summary for CID 54675776, Tetracycline. Retrieved July 12, 2022 from https://pubchem.ncbi.nlm.nih.gov/compound/54675776.

Chopra, Ian, and Marilyn Roberts. "Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance." Microbiology and Molecular Biology Reviews (2001): 232-60. Http://www.ncbi.nlm.nih.gov. Web. 21 Aug. 2012.

Davis R.E. and Whitcomb, 1970, R.F. Evidence on Possible Mycoplasma Etiology of Aster Yellows Disease. Infection and Immunity, Aug. 1970, p. 201-208

Green and Sambrook. Molecular Cloning, A Laboratory Manual (2012).

Backman K, Boyer HW. Tetracycline resistance in Esherichia coli is mediated by one polypeptide. Gene 26: 197-203. (1983).

Gatz, C. Novel inducible/repressible gene expression systems. Methods Cell Biol. 50: 411-424. (1995)

Available Documents

• SDS
• Product Data Sheet
• Susceptibility and Resistance Data

Made by an ISO 9001:2015 certified antibiotic manufacturer focused on creating antibiotics through fermentation.

By removing contamination and streamlining gene selection, these research antibiotics help scientists make discoveries in cell/plant biology, microbiology, virology, stem cell therapy, and cancer research. Specializing in the pharmaceutical and biotech research sectors.

These products are specifically designed for research use and are not approved for human or animal consumption. Not FDA approved.

Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not ship to a registered laboratory or research facility.

Please note: This product is not returnable.