Sigma-Aldrich
Tris-Glycine-SDS Buffer 10X Concentrate, 1 Liter
- Part Number:
- MIL-T7777-1L
- Lead Time:
- 2-4 weeks
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- each
- Size:
- 1 liter / 32 ounce
- Style:
- chemical
- DOT Rated:
- HazMat Transport Fee Required
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Description
Tris-Glycine-SDS Buffer 10X Concentrate, 1 Liter
Synonym: TRIS glycine SDS buffer solution
- eCl@ss 32129211
- NACRES NA.25
| Quality Level | 200 |
| sterility | 0.2 μm filtered |
| Featured Industry | Diagnostic Assay Manufacturing |
| storage temp. | 2-8°C |
General description
Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.
Application
Tris-glycine-SDS buffer 10× concentrate has been used as a running buffer in sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE). It is also used to block membranes with 5% non-fat dry milk for western blotting.
Reconstitution
Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.
Safety Information
Signal word Warning
Hazard statements H319
Precautionary statements P280-P305 + P351 + P338-P337 + P313
Personal Protective Equipment Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
RIDADR NONH for all modes of transport
WGK Germany 1
Documents
Warnings
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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.