Sigma-Aldrich
Trizma Base, Anhydrous Free-Flowing Redi-Dri 99.9% 2.5 kg
- Part Number:
- MIL-RDD008-25KG
- Lead Time:
- 7-14 days
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- Quantity:
- each
- Size:
- 2.5 kg
- Style:
- chemical
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Description
Trizma Baseanhydrous Free-Flowing Redi-Dri ≥99.9% 2.5 Kg
Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
- CAS Number 77-86-1
- Linear Formula NH2C(CH2OH)3
- Molecular Weight 121.14
- Beilstein/REAXYS Number 741883
- EC Number 201-064-4
- MDL number MFCD00004679
- PubChem Substance ID 329824219
- NACRES NA.25
| grade | Redi-Dri |
| anhydrous | |
| description | aminopeptidase substrate |
| assay | ≥99.9% |
| form | powder |
| quality | free-flowing |
| impurities | ≤0.2% Water (Karl Fischer) |
| color | white |
| pH | 10.5-12 |
| pH range | 7 - 9 |
| useful pH range | 7 - 9 |
| pKa (25 °C) | 8.1 |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.) |
| solubility | water: soluble (Water < 0.2%) |
| absorption | ≤0.05 at 290 nm at 40% |
| λ | (A290 UV absorbance < 0.05) |
| in 5 mM Tris; DNA (40% (w/w)) | |
| SMILES string | NC(CO)(CO)CO |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2 |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
General description
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
Application
Tris base has been used:
• To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization
• In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting
• In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein
• As a component of nuclei extraction buffer
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.