Sigma-Aldrich
Dulbecco's Modified Eagle's Medium - High Glucose, 500mL, case/24
- Part Number:
- MIL-D5671-24X500ML
- Lead Time:
- 2-3 weeks
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- 24
- Size:
- 500 ml / 16 ounce
- Style:
- chemical
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Description
Dulbecco’s Modified Eagle’s Medium - High Glucose, 500mL, Case/24
Synonym: DME, DMEM
- NACRES NA.75
| Quality Level | 300 |
| sterility | sterile-filtered |
| form | liquid |
| application(s) | cell culture | hybridoma: suitable |
| cell culture | mammalian: suitable | |
| impurities | endotoxin, tested |
| components | glucose: highNaHCO3: yessodium pyruvate: noL-glutamine: nophenol red: yesHEPES: no |
| shipped in | ambient |
| storage temp. | 2-8°C |
General description
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name ""DMEM/High"".
Safety Information
NONH for all modes of transport
WGK Germany 3
Documents
Warnings
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