DAB Chromogen/Substrate HRP Antibody Conjugation Kit (High Contrast), 500 Slides

DAB Chromogen/Substrate Kit (High Contrast), 500 Slides. This product has been developed for applications that require high contrast between the chromogen and Hematoxylin counterstain. The resulting stain is a darker brown than standard DAB and somewhat more sensitive. ScyTek recommends using Hematoxyling for Automation (catalog # HAQ500) for optimal contrast. 3,3'Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol. This product is available in a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate (High Contrast). The standard working dilution is 50ul (0.9mg) of DAB Chromogen per 1ml of DAB Substrate (High Contrast), although the ratio can be adjusted as desired. The use of liquid components reduces some risks associated with handling powders (ie. dust inhalation), and eliminates waste which often results from using tablets that require a predetermined final volume. Once the two components are combined, the reagent can be used for up to six hours, making it ideal for automated stainers.

• Quantity: 500 Slides
• Storage: 2°C- 8°C
• Download: Instructions For Use
• MSDS: Material Safety Data Sheet

Immunohistochemistry > Chromogens (Immunohistochemistry) > HRP > HRP Antibody Conjugation Kits

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Terms & Conditions

These chemicals are for professional manufacturing, research laboratories and industrial / commercial usage only. Not for medical or consumer use. We currently cannot ship this product to doctor offices, pharmacies, medical facilities, veterinarians or residences. All orders are verified prior to shipment. Orders shipping to consumers or medical facilities will be canceled.

Please note: This product is not returnable. Chemicals cannot be expedited.