DMEM-010: DMEM/F-12 1:1 Mixtures, 1X, with 4500 mg/L D-glucose, 365 mg/L L-glutamine, 325.8 mg/L L-alanyl-L-glutamine, 3700 mg/L sodium bicarbonate, but no HEPES. 500 mL

DMEM-010: DMEM/F-12 1:1 Mixtures, 1X, with 4500 mg/L D-glucose, 365 mg/L L-glutamine, 325.8 mg/L L-alanyl-L-glutamine, 3700 mg/L sodium bicarbonate, but no HEPES. 500 mL. F-12 Nutrient Mixture (Ham's F-12) is a serum-free medium that relies on a combination of nutrient supplements, growth factors, and hormones to culture cells. During the past decade, researchers have reported successful culture of Leydig cells and Sertoli cells in serum-free medium that contained insulin, transferrin, epidermal growth factor (EGF), leutinizing hormone or follicle stimulating hormone, somatomedin and growth hormone. A 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture was found to be most satisfactory for studies of this type. DME-BBZ-5XM-010 contains 4500 mg/L D-glucose, 365 mg/L L-glutamine, 325.8 mg/L L-alanyl-L-glutamine, 3700 mg/L sodium bicarbonate, but no HEPES. The liquid medium should be stored at 2-8°C away from direct sunlight.

Product Type: Cell Culture Media

Product of the USA

Shipping Note: This is a refrigerated item, packed in an insulated cooler box with gel ice. It must be shipped expedited.