Sigma-Aldrich
Kicqstart Probe QPCR Readymix For Bio-Rad Cepheid Eppendorf Illumina Corbett, pack/250
- Part Number:
- MIL-KCQS04-250RXN
- Lead Time:
- 2-3 weeks
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- 250
- Style:
- chemical
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Description
Kicqstart Probe QPCR Readymix For Bio-Rad Cepheid Eppendorf Illumina Corbett Pack/250
Synonym: Kicqstart Probe qPCR ReadyMix
- NACRES NA.55
| form | liquid |
| feature | hotstart |
| storage condition | protect from light |
| color | light blue |
| shipped in | dry ice |
| storage temp. | −20°C |
General description
KiCqStart Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. KiCqStart Probe qPCR ReadyMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.
Application
KiCqStart Probe qPCR ReadyMix has been used for gene expression analysis by qPCR.
Features and Benefits
• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required
Components
2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
Most chemicals cannot be expedited.
This item has a Haz-Mat shipping fee built into the price. Fee is applicable only to the first item purchased. Additional HazMat items will be discounted automatically in the shopping cart. HazMat items always require special DOT handling and in rare cases may incur additional fees not levied prior to checkout. Request a quote for the most accurate pricing.
WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.