Sigma-Aldrich
Minimum Essential Medium Eagle Alpha Modification with Ribonucleosides Deoxyribonucleosides 500mL, case/6
- Part Number:
- MIL-M8042-6X500ML
- Lead Time:
- 2-3 weeks
- Minimum Purchase:
- 1 unit
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- 6
- Size:
- 500 ml / 16 ounce
- Style:
- chemical
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Description
Minimum Essential Medium Eagle Alpha Modification With Ribonucleosides Deoxyribonucleosides 500mL Case/6
Synonym: MEM
- NACRES NA.75
| Quality Level | 300 |
| sterility | sterile-filtered |
| form | liquid |
| application(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| components | glucose: yesstable glutamine: nophenol red: yesEarle’s salts (5% CO2): yessodium pyruvate: yesNaHCO3: yesL-glutamine: noHEPES: noHanks’ salts (2% CO2): no |
| shipped in | ambient |
| storage temp. | 2-8°C |
General description
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Often used in cytogenetic analysis when supplemented with fetal bovine serum (FBS).
Application
Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell, and osteoblast.
Minimum Essential Medium Eagle is used for primary osteoblast cultures and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.
Biochem/physiol Actions
MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.
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Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.