Sigma-Aldrich
B-Nicotinamide Adenine Dinucleotide Reduced Disodium Salt Hydrate 97% HPLC 1 gram
- Part Number:
- MIL-N8129-1G
- Lead Time:
- 2-3 weeks
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- Quantity:
- each
- Size:
- 1 gram
- Style:
- chemical
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Description
β-Nicotinamide Adenine Dinucleotide Reduced Disodium Salt Hydrate ≥97% HPLC 1 Gram
Synonym:β-DPNH, β-NADH, DPNH, Diphosphopyridine nucleotide, reduced form, NADH
- CAS Number 606-68-8 (anhydrous)
- Empirical Formula (Hill Notation) C21H27N7Na2O14P2 · xH2O
- Molecular Weight 709.40 (anhydrous basis)
- EC Number 210-123-3
- MDL number MFCD03791273
- PubChem Substance ID 329818676
- NACRES NA.51
Quality Level | 300 |
assay | ≥97% (HPLC) |
≥97% (spectrophotometric assay) | |
storage temp. | −20°C |
SMILES string | O.[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@@H]2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O |
InChI | 1S/C21H29N7O14P2.2Na.H2O/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33;;;/h1,3-4,7-8,10-11,13-16,20-21,29-32H,2,5-6H2,(H2,23,33)(H,34,35)(H,36,37)(H2,22,24,25);;;1H2/q;2*+1;/p-2/t10-,11-,13-,14-,15-,16-,20-,21-;;;/m1.../s1 |
InChI key | FWBNCIFELNNJCX-UHOVGGJYSA-L |
Application
β-Nicotinamide adenine dinucleotide (NAD+) and β-Nicotinamide adenine dinucleotide, reduced (NADH) comprise a coenzyme redox pair (NAD+:NADH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. In addition to its redox function, NAD+/NADH is a donor of ADP-ribose units in ADP-ribosylaton (ADP-ribosyltransferases; poly(ADP-ribose) polymerases ) reactions and a precursor of cyclic ADP-ribose (ADP-ribosyl cyclases).
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
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