Sigma-Aldrich

Tris(Hydroxymethyl) Aminomethane ACS Reagent, 99.8% 500 grams

Part Number:
MIL-252859-500G
Lead Time:
2-4 weeks
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Quantity:
each
Size:
500 grams
Style:
chemical
Price: $184.29

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Description

Tris(Hydroxymethyl) Aminomethane ACS Reagent 99.8% 500 Gram

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol

  • CAS Number 77-86-1
  • Linear Formula NH2C(CH2OH)3
  • Molecular Weight 121.14
  • Beilstein/REAXYS Number 741883
  • EC Number 201-064-4
  • MDL number MFCD00004679
  • PubChem Substance ID 24855244
  • NACRES NA.25
Grade ACS reagent
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Assay

≥99.8%
99.8-100.1%

impurities

≤0.005% insolubles
≤2% water

useful pH range 7 - 9
pKa (25°C) 8.1
bp 219-220°C/10 mmHg (lit.)
mp 167-172°C (lit.)
cation traces

Fe: ≤5 ppm
heavy metals (as Pb): ≤5 ppm

absorption passes test

General description

Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Application

Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.

Safety Information 

RIDADR NONH for all modes of transport
WGK Germany 1
RTECS TY2900000

Documents

Safety Data Sheet

Warnings

Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.

Most chemicals cannot be expedited.

WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning

Please note: This product is not returnable.

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MPN:
252859-500G