Sigma-Aldrich
Bovine Serum Albumin Fatty Acid Free, 96% Agarose Gel Electrophoresis, 5 grams
- Part Number:
- MIL-A8806-5G
- Lead Time:
- 2-3 weeks
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- each
- Size:
- 5 grams
- Style:
- chemical
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Description
Bovine Serum Albumin Solution 30% ±2% in 0.85% Sodium Chloride Aseptically Filled, 50 mL
Synonym: Albumin bovine serum, BSA, Bovine albumin
- CAS Number 9048-46-8
- EC Number 232-936-2
- MDL number MFCD00130384
- NACRES NA.27
| Quality Level | 300 |
| biological source | bovine |
| product line | BioReagent |
| assay | ≥96% (agarose gel electrophoresis) |
| form | lyophilized powder |
| mol wt | ~66 kDa |
| purified by | cold ethanol fractionation |
| packaging | poly bottle of |
| origin | USA origin |
| free fatty acid content | ≤0.02% |
| application(s) | cell culture | mammalian: suitable |
| impurities | ≤0.02% fatty acid (GC) |
| ≤0.1 ng/mg endotoxin | |
| loss | ≤5% |
| pH | 4.8-7.5 |
| solubility | water: soluble (40 mg/ml) |
| storage temp. | 2-8°C |
General description
Bovine serum albumin (BSA) is a water-soluble serum protein, 583 amino acids in length, with a calculated molecular weight of 66,430 Daltons. Six a-helices form its three homologous domains. Depending on pH, bovine serum albumin undergoes reversible conformational isomerization. BSA native structure becomes reactive and flexible on heating.
BSA is a carrier protein for many biomolecules, such as fatty acids, amino acids, and steroids. For its carrier properties, BSA is also widely used in cell culture. BSA also serves antioxidant functions when used in cell culture.
Application
Select the right albumin for your application with the Albumin Selection Guide
Bovine Serum Albumin has been used in the solution prepared for digestion of tissues. It has been used as a blocking agent in cell spreading assay.
Because BSA is a well-known carrier of fatty acids (FA), control over the particular fatty acids used to culture cell lines is important, for cell culture lines which are sensitive to particular fatty acids. Fatty acid-free BSA is a highly useful reagent for such cell culture experiments, to allow researchers to use such BSA as a carrier for their specific fatty acids specific to their cell culture system. This mitigates potential risks to the cells from FA′s that might be present in non-FA-free BSA.
This BSA product is specifically tested for the following properties:
- Fatty acid free / very low FA content
- Low endotoxin level
- Suitability to use in cell culture
Safety Information
Personal Protective Equipment Eyeshields, Gloves, type N95 (US)
RIDADR NONH for all modes of transport
WGK Germany 3
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
Most chemicals cannot be expedited.
WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.