Sigma-Aldrich
EX-CELL Hybridoma Medium without Antibiotics Antimycotics L-Glutamine And Phenol Red 1 Liter
- Part Number:
- MIL-H4281-1L
- Lead Time:
- 2-3 weeks
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- each
- Size:
- 1 liter / 32 ounce
- Style:
- chemical
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Description
EX-CELL Hybridoma Medium Without Antibiotics Antimycotics L-Glutamine And Phenol Red 1 Liter
Synonym: Hybridoma Medium
- MDL number MFCD03098239
- NACRES NA.75
| Quality Level | 500 |
| description | endotoxin tested |
| for research or for further manufacturing use | |
| sterility | sterile-filtered |
| form | liquid |
| application(s) | cell culture | hybridoma: suitable |
| components | HEPES: yesglucose: 2.9 g/LL-glutamine: nophenol red: noNaHCO3: yes |
| shipped in | ambient |
| storage temp. | 2-8°C |
Application
Serum-free, low-protein medium formulated to support the growth of hybridomas used in the manufacture of monoclonal antibodies.
Features and Benefits
Designed to support the rapid growth of cells to high densities and the maintenance of viable cells for extended culture periods. This formulation reduces raw material cost and the low protein level reduces cost associated with downstream processing. Purified components in the product reduce the variability sometimes encountered with biological materials leading to improved consistency of the medium performance. Cells can be successfully transferred from serum-supplemented cultures to this medium with little or no adaptation.
Other Notes
This proprietary formulation includes inorganic salts, essential and non-essential amino acids, vitamins, sodium bicarbonate, HEPES, trace elements, fatty acids, and other organics. It contains low concentrations of recombinant human insulin, bovine serum albumin and human transferrin (source tested negative for HIV antibody and HbsAG). The medium does not contain L-glutamine, antibiotics, antimycotics, and phenol red.
Caution
Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time. When stored at 2-8°C in the dark, medium that has not been supplemented is stable until the expiration date on the label.
Reconstitution
Prior to use, medium should be warmed to 37°C and supplemented with 50 ml/L of 200 mM L-glutamine.
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
Most chemicals cannot be expedited.
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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.