Sigma-Aldrich
Imidazole For Molecular Biology 99% Titration 25 grams
- Part Number:
- MIL-I5513-25G
- Lead Time:
- 2-4 weeks
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- Quantity:
- each
- Size:
- 25 grams
- Style:
- chemical
- DOT Rated:
- HazMat Transport Fee Required
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Description
Imidazole For Molecular Biology ≥99% Titration 25 Gram
Synonym: 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline
- CAS Number 288-32-4
- Empirical Formula (Hill Notation) C3H4N2
- Molecular Weight 68.08
- Beilstein/REAXYS Number 103853
- EC Number 206-019-2
- MDL number MFCD00005183
- eCl@ss 39161001
- PubChem Substance ID 24896072
- NACRES NA.25
| grade | for molecular biology |
| vapor pressure | |
| assay | ≥99% (titration) |
| form | crystalline powder |
| pH | 6.2-7.8 (25 °C) |
| pKa (25 °C) | 6.95 |
| bp | 256 °C (lit.) |
| mp | 88-91 °C (lit.) |
| Featured Industry | Diagnostic Assay Manufacturing |
| foreign activity | DNase, RNase and protease, none detected |
| SMILES string | c1c[nH]cn1 |
| InChI | 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5) |
| InChI key | RAXXELZNTBOGNW-UHFFFAOYSA-N |
General description
Imidazole is a heterocyclic compound with a five-membered planar ring. It is amphoteric and highly polar. The pharmacophore of imidazole exists in bioactive compounds including amino acids, plant growth regulators and therapeutic agents.
Application
Imidazole has been used
• in the lysis, wash and elution buffer for the purification of histidine tagged Sonic Hedgehog(shh-N) protein
• in elution buffer in stepwise gradient for the purification of histidine tagged aldo keto reductases using nickel affinity chromatography
• as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells
Excellent for buffers in the range of pH 6.2-7.8
GHS05, GHS07, GHS08
Signal word Danger
Hazard statements H302-H314-H360D
Precautionary statements P201-P260-P280-P303 + P361 + P353-P305 + P351 + P338-P308 + P313
Personal Protective Equipment Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
RIDADR UN 3263 8 / PGIII
WGK Germany 2
RTECS NI3325000
Flash Point(F) 293 °F
Flash Point(C) 145 °C
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.