Research Antibiotics, Tazobactam, 5 grams

Tazobactam, 5 gram. Tazobactam is a penicillanic acid sulfone derivative and beta-lactamase inhibitor with antibacterial activity. Tazobactam was discovered by Dr. R.G. Micetech at the University of Alberta in 1982. Tazobactam contains a beta-lactam ring and irreversibly binds to beta-lactamase at or near its active site. This protects other beta-lactam antibiotics from beta-lactamase catalysis. This drug is used in conjunction with beta-lactamase susceptible penicillins to treat infections caused by beta-lactamase producing organisms.

Tazobactam alone showed an MIC of ≤ 8 mg/liter (range 2 to 32 mg/liter) against several Acinetobacter baumannii strains. Tazobactam in combination with piperacillin, successfully restored the activity of piperacillin against β-lactamase-producing bacteria. Tazobactam exhibited inhibitory activity and protected piperacillin against Richmond and Sykes types II, III, IV and V β-lactamases, staphylococcal penicillinase and extended-spectrum β-lactamases. Tazobactam showed species-specific activity against class I chromosomally-mediated enzymes.

• Category: Microbiology
• CAS Number: 89786-04-9
• Molecular Formula: C₁₀H₁₂N₄O₅S
• Molecular Weight: 300.29
• Mechanism of Action: Tazobactam contains a beta-lactam ring that binds strongly to beta-lactamase at or near its activation site, thereby permanently inhibiting enzymatic activity. This action protects other beta-lactam antibiotics (penicillins, cephalosporins, etc.) from beta-lactamase catalysis, thereby enhancing their antibacterial activity.
• Storage Conditions: 2-8°C
• Tariff Code: 2941.10.5000
• Spectrum: azobactam exhibits little useful antimicrobial activity, although weak activity against Acinetobacter spp. and Borrelia burgdorferi has been reported. Tazobactam inhibits a wide range of β-lactamases, including the group 2 penicillinases from Staph. aureus, the TEM-1 and SHV-1 β-lactamases, many extended-spectrum enzymes, and the common group 2e cephalosporinases of B. fragilis. Against the group 1 cephalosporinases, activity is strongly influenced by the amount of enzyme produced. The inhibitor-resistant group 2br β-lactamases are poorly inhibited and group 3 metallo-β-lactamases are not inhibited at clinically useful levels. It is a poor inducer of β-lactamases of Gram-positive and Gram-negative organisms.

Microbiology Applications

Tazobactam is often combined with the extended-spectrum β-lactam antibiotic piperacillin in the drug zosyn or Tazocin (piperacillin/Tazobactam), used in infections due Pseudomonas aeruginosa. Tazobactam broadens the spectrum of piperacillin by making it effective against organisms that express β-lactamase and would normally degrade piperacillin.

Specifications

• Form: powder
• Appearance: White or off-white crystalline powder
• Source: Synthetic
• Water Content (Karl Fischer): Not more than 4.0%
• pH: 2.0-2.5
• Assay (On Dried Basis): Not less than 94.0%
• Heavy Metals: Not more than 20 ppm

References

Craig, W.A., and Andes, D.R. In vivo activities of ceftolozane, a new cephalosporin, with and without Tazobactam against Pseudomonas aeruginosa and Enterobacteriaceae, including strains with extended-spectrum β-lactamases, in the thighs of neutropenic mice. Antimicrob. Agents Chemother. 57(4),1577-1582 (2013).

Wishart, David. "Tazobactam." DrugBank. The Metabolomics Innovation Center, 13 June 2005. Web. 23 Aug. 2012.

Drawz, S.M., and Bonomo, R.A. Three decades of β-lactamase inhibitors. Clin.Microbiol.Rev. 23(1), 160-201 (2010).

Zhanel GG, Chung P, Adam H, et al. Ceftolozane/Tazobactam: a novel cephalosporin/β-lactamase inhibitor combination with activity against multidrug-resistant gram-negative bacilli. Drugs. 2014 Jan;74(1):31-51. PMID: 24352909

Frase H, Smith CA, Toth M, et al. Identification of products of inhibition of GES-2 beta-lactamase by Tazobactam by x-ray crystallography and spectrometry. J Biol Chem. 2011 Apr 22;286(16):14396-409. PMID: 21345789.

Jones, R.N., Pfaller, M.A., Fuchs, P.C., et al. Piperacillin/Tazobactam (YTR 830) combination. Comparative antimicrobial activity against 5889 recent aerobic clinical isolates and 60 Bacteroides fragilis group strains. Diagn. Microbiol. Infect. Dis. 12(6), 489-494 (1989).

Bryson, H.M. and Brogden, R.N. 1994. Drugs. 47: 506-535. PMID: 7514977

Available Documents

• SDS
• Product Data Sheet
• Susceptibility and Resistance Data

Made by an ISO 9001:2015 certified antibiotic manufacturer focused on creating antibiotics through fermentation.

By removing contamination and streamlining gene selection, these research antibiotics help scientists make discoveries in cell/plant biology, microbiology, virology, stem cell therapy, and cancer research. Specializing in the pharmaceutical and biotech research sectors.

These products are specifically designed for research use and are not approved for human or animal consumption. Not FDA approved.

Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not ship to a registered laboratory or research facility.

Please note: This product is not returnable.