Sigma-Aldrich
HEPES 99.5% Titration 500 gram
- Part Number:
- MIL-H3375-500G
- Lead Time:
- 2-3 weeks
- Minimum Purchase:
- 1 unit
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- each
- Size:
- 500 grams
- Style:
- chemical
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Description
HEPES ≥99.5% Titration 500 Gram
Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
- CAS Number 7365-45-9
- Empirical Formula (Hill Notation) C8H18N2O4S
- Molecular Weight 238.30
- Beilstein/REAXYS Number 883043
- EC Number 230-907-9
- MDL number MFCD00006158
- PubChem Substance ID 24895572
- NACRES NA.25
| Quality Level | 300 |
| 400 | |
| assay | ≥99.5% (titration) |
| pH | 5.0-6.5 (25 °C, 238 g/L) |
| useful pH range | 6.8 - 8.2 |
| pKa (25 °C) | 7.5 |
| Featured Industry | Diagnostic Assay Manufacturing |
| SMILES string | OCCN1CCN(CC1)CCS(O)(=O)=O |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
General description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Application
HEPES has been used:
• As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
• As a component of Hanks’ solution used in the degradation testing of Iron
• As a component of HEPES medium in the transfection of cell culture with plasmids
• Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
• As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.