Inhibitor Resistant Genotyping PCR Readymix, pack/250

Inhibitor Resistant Genotyping PCR Readymix Pack/250

Synonym: Inhibitor Resistant Genotyping PCR ReadyMix

• NACRES NA.55

General description

Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.

A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. Inhibitor Resistant Genotyping PCR Readymix is compatible with both fast and conventional PCR cycling protocols.

Application

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:
• Bio-Rad CFX384
• Bio-Rad CFX96
• Bio-Rad MiniOpticon
• Bio-Rad/MJ Chromo4
• Bio-Rad/MJ Opticon 2
• Bio-Rad/MJ Opticon
• Cepheid SmartCycler
• Eppendorf Mastercycler ep realplex
• Eppendorf Mastercycler ep realplex2 s
• Illumina Eco qPCR
• Qiagen/Corbett Rotor-Gene 3000
• Qiagen/Corbett Rotor-Gene 6000
• Qiagen/Corbett Rotor-Gene Q
• Roche LightCycler 480

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.

Documents 

Warnings

Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.

Most chemicals cannot be expedited.

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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning

Please note: This product is not returnable.