Research Antibiotics, Trimethoprim, 25 grams

Trimethoprim, 25 gram. Trimethoprim lactate is a synthetic derivative of trimethoxybenzyl-pyrimidine with bacteriostatic and antiprotozoal properties. As a pyrimidine inhibitor of bacterial dihydrofolate reductase, trimethoprim binds tightly to the bacterial enzyme, blocking the production of tetrahydrofolic acid from dihydrofolic acid, arresting folic acid synthesis.

When Trimethroprim lactate is combined with sulfonamides (like sulfamethoxazole (S045))the two compounds show bactericidal effects, but are only bacteriostatic when used separately. The activity is attributed to their synergistic effect in inhibiting folic acid metabolism in bacteria.

Trimethoprim lactate has a wide antibacterial spectrum and is active against most gram-positive and gram-negative aerobic bacteria, including Nocardia, Brucella, Gram-negative bacilli, and some Gram-positive bacteria like Streptococcus, Toxoplasma and some other coccidians. It is used to treat recurrent cystitis, mild acute prostatitis, urinary tract infections, asymptomatic bacteriuria during pregnancy and respiratory tract infections.

Trimethoprim is slightly soluble in aqueous solution (12.1 mg/mL). Trimethoprim lactate is freely soluble in aqueous solution (19.6 mg/mL).

• Category: Microbiology
• CAS Number: 738-70-5
• Molecular Formula: C14H18N4O3
• Molecular Weight: 290.32
• Mechanism of Action: Trimethoprim interferes with the cellular metabolism of folic acid in the bacterial cell by blocking the biosynthesis of nucleotides. Trimethoprim binds to dihydrofolate reductase and inhibits the reduction of dihydrofolic acid to tetrahydrofolic acid. Tetrahydrofolic acid is an essential precursor in the thymidine synthesis pathway and interference with this pathway inhibits bacterial DNA synthesis.
• Storage Conditions: 2-8°C
• Tariff Code: 2933.59.2200
• Spectrum: Trimethoprim lactate has a wide antibacterial spectrum and is active against most gram-positive and gram-negative aerobic bacteria, including Nocardia, Brucella, Gram-negative bacilli, and some Gram-positive bacteria like Streptococcus, Toxoplasma and some other coccidians. It is used to treat recurrent cystitis, mild acute prostatitis, urinary tract infections, asymptomatic bacteriuria during pregnancy and respiratory tract infections.

Microbiology Applications

Trimethoprim lactate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

Escherichia coli 0.25 µg/mL - 64 µg/mL
Haemophilus influenzae 0.15 µg/mL - 16 µg/mL

Media Supplement

Trimethoprim can be used as a selective agent in several types of isolation media:

• Columbia Blood Agar - Campylobacter selective supplement (Skirrow)
• Columbia Blood Agar - Campylobacter selective supplement (Blaser-Wang)
• Campylobacter Agar - Campylobacter Selective Supplement (Preston)
• Columbia Blood Agar - Helicobacter pylori Selective Supplement (Dent)
• Bolton Broth - Bolton Broth Selective Supplement
• Campylobacter Agar Base - Modified Preston Campylobacter Selective Supplement
• Bolton Broth - Modified Bolton Broth Selective Supplement
• Chromogenic Bacillus cereus Agar - Chromogenic Bacillus cereus Selective Supplement

Plant Biology Applications

Trimethoprim lactate can be used in combination with rifampicin to provide sufficient coverage against pathogenic microbes. When used without other supplemental antibiotics, trimethoprim was not shown to provide sufficient coverage (Pollock et al.1983).

Specifications

• Form: powder
• Appearance: white to yellowish-white powder
• Melting Point: 199°C - 203°C
• Assay (on dried basis): 98.5 - 101.0%
• Loss on Drying: Not more than 0.5%
• Residue On Ignition: Not more than 0.1%

References

Weir, D. G., and J. Scott. "Mechanism of the Antimicrobial Drug Trimethoprim Revisited. "PubMed (2000): 2519-524. www.ncbi.gov. 14 Dec. 2000. Web. 21 Aug. 2012.

Kneifel W. and Leonhardt W., Testing of different antibiotics against Gram-positive and Gram-negative bacteria isolated from plant tissue culture. Plant Cell, Tissue and Organ Culture, Vol. 29, pp. 139-144, 1992.

Pollock K., Barfield D.G. and Shields R., The toxicity of antibiotics to plant cell cultures. Plant cell reports, Vol. 2, pp. 36-39, 1983.

Available Documents

• SDS
• Product Data Sheet
• Susceptibility and Resistance Data

Made by an ISO 9001:2015 certified antibiotic manufacturer focused on creating antibiotics through fermentation.

By removing contamination and streamlining gene selection, these research antibiotics help scientists make discoveries in cell/plant biology, microbiology, virology, stem cell therapy, and cancer research. Specializing in the pharmaceutical and biotech research sectors.

These products are specifically designed for research use and are not approved for human or animal consumption. Not FDA approved.

Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not ship to a registered laboratory or research facility.

Please note: This product is not returnable.