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Tris(Hydroxymethyl) Aminomethane Puriss, 99.7%, 100 Gram

Tris(Hydroxymethyl) Aminomethane Puriss, 99.7%, 100 Gram

$85.00
Part Number:
MIL-93350-100G
Lead Time:
2-3 weeks
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Size:
100 gram

Description

Tris(Hydroxymethyl) Aminomethane Puriss, 99.7%, 100 Gram

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1
  • Linear Formula NH2C(CH2OH)3
  • Molecular Weight 121.14
  • Beilstein/REAXYS Number 741883
  • EC Number 201-064-4
  • MDL number MFCD00004679
  • PubChem Substance ID 57653537
  • NACRES NA.25

 

description   aminopeptidase substrate
grade   puriss. p.a.
assay   ≥99.7% (T)
form   crystalline
ign. residue   ≤0.01% (as SO4)
pH   10.5-11.5 (25 °C, 10% in H2O)
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg (lit.)
mp   167-172 °C (lit.)
  168-172 °C
solubility   water: soluble (678 g/l at 20 °C)
anion traces   chloride (Cl-): ≤5 mg/kg
  sulfate (SO42-): ≤5 mg/kg
cation traces   Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   10 % in H2O
UV absorption   λ: 230 nm Amax: ≤0.6
  λ: 260 nm Amax: ≤0.05
  λ: 280 nm Amax: ≤0.04
  λ: 430 nm Amax: ≤0.01
SMILES string   NC(CO)(CO)CO
InChI   1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key   LENZDBCJOHFCAS-UHFFFAOYSA-N

General description
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application
Tris base has been used:
• In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
• As a component of sample buffer for SDS-PAGE[4]
• In the preparation of IEC (ion-exchange chromatography) buffer

 

Safety Information

Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves
RIDADR NONH for all modes of transport
WGK Germany 1
RTECS TY2900000

Documents

Safety Data Sheet

Warnings

Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required. 

Most chemicals cannot be expedited.

WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning

Please note: This product is not returnable.

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Product Reviews

MPN:
93350-100G