Accuris qMAX Probe One-Step 4X RT-qPCR Kit, Choose Quantity

AccurisTM qMAX Probe 1-Step 4X RT-qPCR Mix, No ROX

Accuris™ qMAX™ Probe 1-Step 4× RT-qPCR Mix is a high-concentration, one-step reverse transcription quantitative PCR system optimized for sensitive detection of viral RNA and DNA targets. The system comprises a 4× qMAX™ Probe Master Mix and a separate 20× qMAX™ Reverse Transcriptase supplemented with an RNase inhibitor, enabling modular assay design and improved reagent utilization.

The 4× master mix format permits increased template input per reaction, enhancing analytical sensitivity for low-copy targets. Additionally, the elevated concentration supports reduced total reaction volumes, facilitating rapid thermal cycling while maintaining amplification efficiency and reproducibility. The formulation is specifically optimized for dual-labeled hydrolysis probe-based assays and is compatible with both standard and fast cycling protocols. It supports robust performance in singleplex and multiplex RT-qPCR applications, including higher-order multiplexing (>3 targets).Buffer composition and enzyme system are engineered to maintain amplification efficiency across a broad range of template complexities, including GC-rich and AT-rich regions, while exhibiting tolerance to common PCR inhibitors.

To maximize compatibility with multiplex assay designs and diverse instrumentation, the master mix is supplied without ROX passive reference dye, allowing user-defined normalization strategies where required.

Highlights:

• One-step RT-qPCR system for sensitive detection of viral RNA and DNA targets
• 4× formulation enables increased template input, improving low-copy target detection
• Optimized for dual-labeled hydrolysis probe-based assays
• Compatible with both standard and fast RT-qPCR cycling protocols
• Robust amplification across GC-rich and AT-rich templates
• Demonstrates tolerance to common PCR inhibitors

Storage:

Upon receipt, immediately store at -20°C. Avoid excessive freeze/thaw cycles. When stored as directed, this product will retain its activity for 12 months from date of receipt.

Limitation of Use:

For research purposes only. Not intended for therapeutic or diagnostic use.

Quality Control:

Accuris enzymes and reagents are tested under general assay conditions for activity, reproducibility, efficiency, heat activation, sensitivity, and absence of nuclease contamination and nuclease activity. This product is manufactured under a comprehensive quality management system, following ISO 9001:2008 standards.

PCR Master Mix Guidelines:

qPCR Cycling Conditions:

General Guidelines:

1. Primer Design: Design primers using dedicated tools such as Primer3 or Visual OMP. Aim for primer melting temperatures (Tm) near 60 °C, and select a probe with a Tm approximately 6–10 °C higher. When working with TaqMan™ probes, place the probe in close proximity to the 5′ primer and avoid ending the probe sequence with a guanine base.
2. Amplicon Size: Target amplicons are typically between 80 and 200 base pairs in length, and in most cases should remain under 400 base pairs.
3. ROX requirement: Requirements for ROX™ passive reference dye vary by real-time PCR platform. Many newer instruments no longer depend on ROX, though they may still support it for signal normalization. qMAX Probe 1-Step 4× RT-qPCR Mix is formulated without ROX.

Choose reaction volumes from the drop-down menu: