Sigma-Aldrich
Dl-Dithiothreitol 98% HPLC 99% Titration, 10 grams
- Part Number:
- MIL-D9779-10G
- Lead Time:
- 2-4 weeks
- Minimum Purchase:
- 1 unit
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- each
- Size:
- 10 grams
- Style:
- chemical
- DOT Rated:
- HazMat Transport Fee Required
Bulk discount rates
Automatically save when you buy in bulk.
| Buy 6 - 11 | and get 10% off |
| Buy 12 or above | and get 15% off |
Description
Dl-Dithiothreitol ≥98% HPLC ≥99% Titration 10 Gram
Synonym: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
- CAS Number 3483-12-3
- Linear Formula HSCH2CH(OH)CH(OH)CH2SH
- Molecular Weight 154.25
- Beilstein/REAXYS Number 1719757
- EC Number 222-468-7
- MDL number MFCD00004877
- PubChem Substance ID 24894330
- NACRES NA.31
| grade | for molecular biology |
| assay | ≥98% (HPLC) |
| ≥99% (titration) | |
| mp | 41-44 °C (lit.) |
| solubility | H2O: 50 mg/mL |
| cation traces | heavy metals (as Pb): ≤5 ppm |
| foreign activity | DNase, RNase, protease, none detected |
| storage temp. | 2-8°C |
| SMILES string | O[C@H](CS)[C@H](O)CS |
| InChI | 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1 |
| InChI key | VHJLVAABSRFDPM-QWWZWVQMSA-N |
Application
DL-Dithiothreitol has been added to the samples before loading them for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It has been used in the procedure of in situ hybridization of mRNA.
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
GHS07
Signal word Warning
Hazard statements H302-H412
Precautionary statements P264-P270-P273-P301 + P312 + P330-P501
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves
RIDADR UN 3335
WGK Germany 3
RTECS EK1610000
Flash Point(F) 235.4 °F
Flash Point(C) 113 °C
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
Most chemicals cannot be expedited.
This item has a Haz-Mat shipping fee built into the price. Fee is applicable only to the first item purchased. Additional HazMat items will be discounted automatically in the shopping cart. HazMat items always require special DOT handling and in rare cases may incur additional fees not levied prior to checkout. Request a quote for the most accurate pricing.
WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.