Sigma-Aldrich
Isopropyl B-D-1-thiogalactopyranoside 99% TLC, 10 grams
- Part Number:
- MIL-I5502-10G
- Lead Time:
- 2-4 weeks
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- Quantity:
- each
- Size:
- 10 grams
- Style:
- chemical
- DOT Rated:
- HazMat Transport Fee Required
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Description
Isopropyl Β-D-1-Thiogalactopyranoside ≥99% Tlc 10 Gram
Synonym: IPTG, Isopropyl β-D-thiogalactoside
- CAS Number 367-93-1
- Empirical Formula (Hill Notation) C9H18O5S
- Molecular Weight 238.30
- Beilstein/REAXYS Number 4631
- EC Number 206-703-0
- MDL number MFCD00063273
- PubChem Substance ID 24896069
- NACRES NA.31
| description | non-ionic |
| assay | ≥99% (TLC) |
| form | powder |
| application(s) | thin layer chromatography (TLC): suitable |
| impurities | 10.0-22.0% Dioxane |
| storage temp. | 2-8°C |
| SMILES string | CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O |
| InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 |
| InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
Application
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Other Notes
Non-metabolizable galactose analog.
Reconstitution
A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.
GHS07, GHS08
Signal word Warning
Hazard statements H319-H351
Precautionary statements P281-P305 + P351 + P338
RIDADR NONH for all modes of transport
WGK Germany 3
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
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