Jumpstart Redtaq Readymix Reaction Mix For PCR, pack/100

Jumpstart Redtaq Readymix Reaction Mix For PCR Pack/100

• MDL number MFCD00166026

General description

JumpStart REDTaq ReadyMix PCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma′s ReadyMix and REDTaq. The mix includes Sigma′s JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2× optimized reaction concentrate. Add 25 μL of the ReadyMix to primers, template, and water to a final reaction volume of 50 μL.

JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored.

The red dye allows quick visual confirmation the enzyme has been added and properly mixed. After PCR, an aliquot may be loaded directly onto an agarose gel without the addition of loading buffers. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired.

Using JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.

Application

Jumpstart^™ REDTaq ReadyMix^™ Reaction Mix is used for direct DNA extraction and amplification using PCR.
• For PCR amplifications that require reduced non-specific amplification
• For multiplex PCR
• For reduction of primer dimers
• for conventional PCR amplification
• Methylation-specific PCR (MSPCR)
• Reverse transcription PCR

Features and Benefits

• JumpStart Taq DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
• JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration
• REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications
• Inert red dye allows for easy verification that reagent has been mixed properly
• Other loading dyes not necessary. Aliquots may be directly loaded onto an agarose gel after the reaction

GHS07

Signal word Warning
Hazard statements H317
Precautionary statements P280
RIDADR NONH for all modes of transport
WGK Germany 3

Documents 

Warnings

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WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning

Please note: This product is not returnable.