Sigma-Aldrich
Mes Monohydrate Bioxtra 99% T 500 gram
- Part Number:
- MIL-69892-500G
- Lead Time:
- 2-4 weeks
- Shipping:
- FREE SHIPPING on most orders over $50*
- Quantity:
- each
- Size:
- 500 grams
- Style:
- chemical
- DOT Rated:
- HazMat Transport Fee Required
Bulk discount rates
Automatically save when you buy in bulk.
| Buy 6 - 11 | and get 10% off |
| Buy 12 or above | and get 15% off |
Description
Mes Monohydrate Bioxtra ≥99.0% T 500 Gram
Synonym: 2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid monohydrate
- CAS Number 145224-94-8
- Empirical Formula (Hill Notation) C6H13NO4S · H2O
- Molecular Weight 213.25
- Beilstein/REAXYS Number 6350956
- EC Number 224-632-3
- MDL number MFCD00149409
- eCl@ss 32129211
- PubChem Substance ID 57652221
- NACRES NA.25
| product line | BioXtra |
| assay | ≥99.0% (T) |
| useful pH range | 5.5 - 6.7 |
| pKa (25 °C) | 6.1 |
| mp | >300 °C (lit.) |
| solubility | H2O: 0.5 M at 20 °C, clear |
| anion traces | chloride (Cl-): ≤50 mg/kg |
| sulfate (SO42-): ≤50 mg/kg | |
| cation traces | Ca: ≤50 mg/kg |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| Featured Industry | Diagnostic Assay Manufacturing |
| SMILES string | O.OS(=O)(=O)CCN1CCOCC1 |
| InChI | 1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2 |
| InChI key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
General description
MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.
Application
MES Monohydrate has been used:
• To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay
• As a buffering agent to stabilize enzymatic solutions
• As a component of PAGE running buffer
Preparation Note
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
Other Notes
Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.
dust mask type N95 (US), Eyeshields, Gloves
RIDADR NONH for all modes of transport
WGK Germany 1
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
Most chemicals cannot be expedited.
This item has a Haz-Mat shipping fee built into the price. Fee is applicable only to the first item purchased. Additional HazMat items will be discounted automatically in the shopping cart. HazMat items always require special DOT handling and in rare cases may incur additional fees not levied prior to checkout. Request a quote for the most accurate pricing.
WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.