Sigma-Aldrich
Tris(Hydroxymethyl) Aminomethane Puriss, 99.7%, 500 gram
- Part Number:
- MIL-93350-500G
- Lead Time:
- 2-4 weeks
- Minimum Purchase:
- 1 unit
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- Quantity:
- each
- Size:
- 500 grams
- Style:
- chemical
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Description
Tris(Hydroxymethyl) Aminomethane Puriss, 99.7%, 500 Gram
Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
- CAS Number 77-86-1
- Linear Formula NH2C(CH2OH)3
- Molecular Weight 121.14
- Beilstein/REAXYS Number 741883
- EC Number 201-064-4
- MDL number MFCD00004679
- PubChem Substance ID 57653537
- NACRES NA.25
| description | aminopeptidase substrate |
| grade | puriss. p.a. |
| assay | ≥99.7% (T) |
| form | crystalline |
| ign. residue | ≤0.01% (as SO4) |
| pH | 10.5-11.5 (25 °C, 10% in H2O) |
| useful pH range | 7 - 9 |
| pKa (25 °C) | 8.1 |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.) |
| 168-172 °C | |
| solubility | water: soluble (678 g/l at 20 °C) |
| anion traces | chloride (Cl-): ≤5 mg/kg |
| sulfate (SO42-): ≤5 mg/kg | |
| cation traces | Ca: ≤10 mg/kg |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| λ | 10 % in H2O |
| UV absorption | λ: 230 nm Amax: ≤0.6 |
| λ: 260 nm Amax: ≤0.05 | |
| λ: 280 nm Amax: ≤0.04 | |
| λ: 430 nm Amax: ≤0.01 | |
| SMILES string | NC(CO)(CO)CO |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2 |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
General description
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
Application
Tris base has been used:
• In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
• As a component of sample buffer for SDS-PAGE[4]
• In the preparation of IEC (ion-exchange chromatography) buffer
Safety Information
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves
RIDADR NONH for all modes of transport
WGK Germany 1
RTECS TY2900000
Documents
Warnings
Note: These chemicals are for research & laboratory use only. Your order will not be processed if you do not work for, and ship to, a registered laboratory or research facility. Not for topical applications. An official Declaration of Use form may be required.
Most chemicals cannot be expedited.
WARNING: This product can expose you to chemicals which are known to the State of California to cause cancer and reproductive harm. Prop 65 Warning
Please note: This product is not returnable.